This document provides data and code for analyzing and generating graphs related to the E. coli pulse/saturation project. The provided MATLAB and Python codes are designed for pulse feeding analysis and were written in MATLAB R2015b. If you use any of the data or code, please cite the following paper: Karthik Sekar, Roberto Rusconi, John T Sauls, Tobias Fuhrer, Elad Noor, Jen Nguyen, Vicente I Fernandez, Marieke F Buffing, Michael Berney, Suckjoon Jun, Roman Stocker, Uwe Sauer. Molecular Systems Biology, 2018; 14(11): e8623. Published on November 5, 2018. DOI: 10.15252/msb.20188623.

The repository is divided into the following sections:

Part 1 - Data and Analysis of Lag Time vs. Feeding Frequency

This section contains all the OD data and code for the wild-type pulse experiments. The code generates the following graphs:

- All OD vs. Time plots with linear threshold fitting.

- Relationship between lag time, feeding rate, and linear growth rate.

- Summary table for the total glucose needed for proliferation and feeding speed.

Part 2 - Flow Cytometry